1YPQ

Human Oxidized Low Density Lipoprotein Receptor LOX-1 Dioxane Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-12
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	70.700, 49.100, 76.300
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	9.960 - 1.400
R-factor	0.177
Rwork	0.177
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mpu 1k9i 1q03
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge		0.270
Number of reflections	50675
<I/σ(I)>	25	2.4
Completeness [%]	99.0	91.8
Redundancy	4.1	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	277	PEG10K, Bicine, Dioxane, HEPES, NaCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K