1YOX
Structure of the conserved Protein of Unknown Function PA3696 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-26 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98043,0.98027,0.96517 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.912, 124.912, 164.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.22732 |
| Rwork | 0.225 |
| R-free | 0.27896 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.044 | 0.480 |
| Number of reflections | 58292 | |
| <I/σ(I)> | 27.03 | 2.93 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.6 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.5M MgCl2, 0.1M Tris pH 8.5, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 8.50 |
