1YOL
Crystal structure of Src kinase domain in complex with CGP77675
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.530, 62.950, 72.790 |
| Unit cell angles | 100.38, 89.16, 89.99 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19585 |
| Rwork | 0.193 |
| R-free | 0.24746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FMK/3LCK hybrid |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.688 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.203 |
| Number of reflections | 29676 | |
| <I/σ(I)> | 9.9 | 5.2 |
| Completeness [%] | 96.0 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 20% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
