1YOE
Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.466, 84.062, 112.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.780 |
| R-factor | 0.16811 |
| Rwork | 0.167 |
| R-free | 0.19276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | YeiK PDB code 1Q8F |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 34056 | |
| <I/σ(I)> | 26.2 | 2.9 |
| Completeness [%] | 97.8 | 77.4 |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | MPD, Sodium Acetate, ribose, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
