1YNY
Molecular Structure of D-Hydantoinase from a Bacillus sp. AR9: Evidence for mercury inhibition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 2000-11-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 64 |
Unit cell lengths | 129.540, 129.540, 102.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.199 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Modified D-hydantoinase from Thermus sp. (PDB ID 1GKP) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.339 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.376 | |
Number of reflections | 42440 | |
Completeness [%] | 97.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | PEG 3350, Cesium Chloride, imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |