1YNA

ENDO-1,4-BETA-XYLANASE, ROOM TEMPERATURE, PH 4.0

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	298
Collection date	1995-04-24
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	40.960, 52.570, 50.470
Unit cell angles	90.00, 100.43, 90.00

Refinement procedure

Resolution	25.300 - 1.550
R-factor	0.155 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	CCP4
Data scaling software	XDS
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.300	1.590
High resolution limit [Å]	1.550	1.550
Rmerge	0.075	0.354
Number of reflections	26211
<I/σ(I)>	7.7
Completeness [%]	83.9	30.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	4 *	5-10% PEG-4000, PH 4.0, T=4 DEG C, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	PEG6000	2.5-5.0 (%(w/v))
3	1	drop	n-octyl-beta-glycoside	0.1 (%(w/v))
4	1	drop	citrate	100 (mM)
5	1	reservoir	PEG6000
6	1	reservoir	citrate