1YN4

Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	93
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9199
Spacegroup name	P 32
Unit cell lengths	38.550, 38.550, 69.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.030 - 1.800
R-factor	0.233
Rwork	0.233
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.870
High resolution limit [Å]	1.800	1.800
Number of reflections	10167
Completeness [%]	99.1	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K