1YMT
Mouse SF-1 LBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03326 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.853, 73.853, 117.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.090 - 1.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PK5 (mLRH-1 LBD) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.320 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.061 | |
| Number of reflections | 95280 | |
| <I/σ(I)> | 38 | |
| Completeness [%] | 94.0 | 100 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 8 mg/ml mSF-1, 6X molar excess of mSHP peptide, 0.1 M Tris, 14% PEG 8000, 5% isopropanol, 15% xylitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
