Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.115870 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 110.531, 110.531, 53.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.260 - 2.240 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j4n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.041 | 0.500 |
| Number of reflections | 16284 | |
| <I/σ(I)> | 27.96 | 2.6 |
| Completeness [%] | 93.7 | 80 |
| Redundancy | 20.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 298 | 30% polyethylene glycol 1000, 20 mM glycine, 50 mM NaCl, pH 10.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
