1YKW
Crystal Structure of a Novel RuBisCO-Like Protein from the Green Sulfur Bacterium Chlorobium tepidum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1271 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.348, 78.451, 90.369 |
Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
Resolution | 87.710 - 2.000 |
R-factor | 0.20329 |
Rwork | 0.201 |
R-free | 0.24539 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5rub |
RMSD bond length | 0.013 |
RMSD bond angle | 1.626 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 60951 | |
<I/σ(I)> | 7.7 | 2 |
Completeness [%] | 97.5 | 84.7 |
Redundancy | 3.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 3350, magnesium formate , pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |