1YK3

Crystal structure of Rv1347c from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2002-03-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	75.665, 77.261, 296.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.960 - 2.200
R-factor	0.227
Rwork	0.227
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.250
High resolution limit [Å]	2.150	2.150
Rmerge	0.092	0.551
Number of reflections	96453
<I/σ(I)>	21.2	3.1
Completeness [%]	99.9	100
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Methoxy, PEG 5000, Tris-HCl, beta-octylglucoside, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Methoxy, PEG 5000, Tris-HCl, beta-octylglucoside, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K