1YJO
Structure of NNQQNY from yeast prion Sup35 with zinc acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 21.153, 4.870, 23.130 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 22.540 - 1.300 |
| R-factor | 0.103 |
| Rwork | 0.102 |
| R-free | 0.15200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 2166 | |
| <I/σ(I)> | 9.8 | 2.6 |
| Completeness [%] | 97.1 | 88.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | ZINC SULFATE, SODIUM ACETATE, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00 |
