1YJD
Crystal structure of human CD28 in complex with the Fab fragment of a mitogenic antibody (5.11A1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.219, 47.417, 71.842 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.248 |
| Rwork | 0.239 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.150 | |
| Number of reflections | 17413 | |
| Completeness [%] | 96.8 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 294 | PEG 3350, magnesium formate, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
