1YJ7

Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 65
Unit cell lengths	164.817, 164.817, 67.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.200 - 1.800
R-factor	0.185
Rwork	0.184
R-free	0.20600
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.232
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.170	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.082	0.313
Number of reflections	96428
<I/σ(I)>		4.6
Completeness [%]	99.9	99.9
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.2	291	di-ammonium hydrogen phosphate, pH 8.2, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	8.2	291	di-ammonium hydrogen phosphate, pH 8.2, VAPOR DIFFUSION, temperature 291K