1YIP

Oxidized Peptidylglycine Alpha-Hydroxylating Monooxygenase (PHM) in a New Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	58.523, 65.683, 69.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.010 - 2.200
R-factor	0.192
Rwork	0.190
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1opm
RMSD bond length	0.018
RMSD bond angle	1.822
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_5.0)
Phasing software	AMoRE
Refinement software	REFMAC (refmac_5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.010	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.058	0.444
Total number of observations		9440
Number of reflections	14064
<I/σ(I)>	12.04	2.18
Completeness [%]	92.9	100
Redundancy	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG 4000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K