1YI6
C-term tail segment of human tyrosine kinase (258-533)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-09-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.985, 63.421, 76.693 |
| Unit cell angles | 90.00, 109.54, 90.00 |
Refinement procedure
| Resolution | 19.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2src |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.235 |
| Number of reflections | 37400 | |
| <I/σ(I)> | 8.8 | 3.1 |
| Completeness [%] | 95.4 | 93.1 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
