1YHS

Crystal structure of Pim-1 bound to staurosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	97.728, 97.728, 80.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.440 - 2.150
R-factor	0.233
Rwork	0.205
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.700
Data scaling software	d*TREK (7.1SSI)
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.580	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	22615
Completeness [%]	94.9	74.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	ammonium phosphate, citrate buffer, NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K