1YHM
Structure of the complex of Trypanosoma cruzi farnesyl disphosphate synthase with alendronate, Isopentenyl diphosphate and mg+2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 103.205, 103.205, 385.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.710 - 2.500 |
| R-factor | 0.2114 |
| Rwork | 0.208 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yhk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.981 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.146 | 0.895 |
| Number of reflections | 55148 | |
| Completeness [%] | 95.6 | |
| Redundancy | 12.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.6 | 293 | peg 4000 Na acetate amonium sulfate, pH 4.6, LIQUID DIFFUSION, temperature 293K |
