1YHF
Crystal Structure of Conserved SPY1581 Protein of Unknown Function from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-02 |
| Detector | SBC-3 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 39.897, 39.897, 144.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.137 | 0.746 |
| Number of reflections | 8535 | |
| <I/σ(I)> | 33.6 | 2.62 |
| Completeness [%] | 99.1 | 94.8 |
| Redundancy | 23.4 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 30% PEG 3000, 0.1 M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.50 |
