1YGT

Dynein Light Chain TcTex-1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.99187, 0.97950, 0.97897, 0.96672
Spacegroup name	P 32 2 1
Unit cell lengths	60.598, 60.598, 48.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.2
Rwork	0.193
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.055	0.198
Number of reflections	10932
<I/σ(I)>	37.6	12
Completeness [%]	93.8	96.9
Redundancy	8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K
1	VAPOR DIFFUSION	9.5	293	0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K
1	VAPOR DIFFUSION	9.5	293	0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K