1YFU

Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from Ralstonia metallidurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	58.100, 58.100, 230.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.110 - 1.900
R-factor	0.216
Rwork	0.216
R-free	0.24000
Structure solution method	SAD + MR
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.110	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	17369
Completeness [%]	90.3	72.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	PEG 8000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	PEG 8000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K