1YFQ
High resolution S. cerevisiae Bub3 mitotic checkpoint protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.274, 74.089, 94.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.100 |
| R-factor | 0.152 |
| Rwork | 0.152 |
| R-free | 0.18600 |
| Structure solution method | MIR |
| RMSD bond length | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.043 | 0.422 |
| Number of reflections | 137806 | |
| <I/σ(I)> | 18.7 | 3.05 |
| Completeness [%] | 97.8 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 8000, Ca acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
