1YFM

RECOMBINANT YEAST FUMARASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1994-03
Detector	SIEMENS
Spacegroup name	P 42 21 2
Unit cell lengths	95.190, 95.190, 100.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.196
Rwork	0.196
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fuo
RMSD bond length	0.014
RMSD bond angle	21.940 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.620
High resolution limit [Å]	2.590	2.590
Rmerge	0.109
Total number of observations	118256 *
Number of reflections	14127
<I/σ(I)>	6.9	0.487
Completeness [%]	94.5	66.7 *
Redundancy	7.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.3		150 MM TRIS-HCL PH 8.3, 100 MM LI2SO4, 8-10% PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	150 (mM)
2	1	1		100 (mM)
3	1	1	PEG4000	8-10 (%)