1YFI
Crystal Structure of restriction endonuclease MspI in complex with its cognate DNA in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-08 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9040 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.295, 111.266, 130.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.940 - 2.700 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sa3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.072 | 0.282 |
Number of reflections | 21426 | |
<I/σ(I)> | 28.7 | 7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MES, ammonium sulfate, calcium chloride, PEG 8000, glycerol , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | ammonium sulfate | ||
3 | 1 | 1 | PEG 8000 | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 1 | water | ||
6 | 1 | 2 | MES | ||
7 | 1 | 2 | ammonium sulfate | ||
8 | 1 | 2 | PEG 8000 | ||
9 | 1 | 2 | glycerol |