1YF5
Cyto-Epsl: The Cytoplasmic Domain Of Epsl, An Inner Membrane Component Of The Type II Secretion System Of Vibrio Cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.006, 88.707, 55.371 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.21553 |
| Rwork | 0.213 |
| R-free | 0.26881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w97 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 7122 | |
| <I/σ(I)> | 15.1 | 3.4 |
| Completeness [%] | 95.8 | 81 |
| Redundancy | 7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG 6000, 150mM calcium acetate, 100mM tris pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
