1YEY

Crystal Structure of L-fuconate Dehydratase from Xanthomonas campestris pv. campestris str. ATCC 33913

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-12
Detector	MARRESEARCH
Wavelength(s)	0.97934, 0.97911, 0.97166
Spacegroup name	P 32 2 1
Unit cell lengths	130.527, 130.527, 193.749
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.340
R-factor	0.249
Rwork	0.247
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.420
High resolution limit [Å]	2.340	2.340
Number of reflections	78328
Completeness [%]	96.8	93.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	PEG 3350, magnesium formate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K