1YER

HUMAN HSP90 GELDANAMYCIN-BINDING DOMAIN, "CLOSED" CONFORMATION

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-11-01
Detector	RIGAKU
Spacegroup name	I 2 2 2
Unit cell lengths	65.587, 88.546, 99.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.650
R-factor	0.194
Rwork	0.194
R-free	0.24400
RMSD bond length	0.007
RMSD bond angle	1.600
Data reduction software	HKL
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.650 *
Rmerge	0.038
Total number of observations	361958 *
Number of reflections	34516
Completeness [%]	98.2
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	PEG8000	30 (%(w/v))