1YED

STRUCTURE OF A CATALYTIC ANTIBODY IGG2A FAB FRAGMENT (D2.4)

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-09-12
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	99.250, 104.790, 223.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 3.100
R-factor	0.215
Rwork	0.215
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1YEC FOR THE VARIABLE DOMAINS AND PDB ENTRY 1DFB FOR THE CONSTANT DOMAINS
RMSD bond length	0.008
RMSD bond angle	28.020 *
Data reduction software	MOSFLM (5.2)
Data scaling software	CCP4
Phasing software	X-PLOR (3.84)
Refinement software	X-PLOR (3.84)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.100
Rmerge	0.071
Number of reflections	18722
Completeness [%]	87.6
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		pH 7.0