1YDO
Crystal Structure of the Bacillis subtilis HMG-CoA Lyase, Northeast Structural Genomics Target SR181.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.693, 104.666, 97.445 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.710 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.30300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.377 |
| Number of reflections | 61067 | |
| <I/σ(I)> | 15.11 | 3.66 |
| Completeness [%] | 95.8 | |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG3350, 100 mM NaI, and 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
