1YDE
Crystal Structure of Human Retinal Short-Chain Dehydrogenase/Reductase 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 167.115, 98.823, 167.463 |
Unit cell angles | 90.00, 115.87, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.18166 |
Rwork | 0.181 |
R-free | 0.22903 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.505 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.117 | 0.706 |
Number of reflections | 176307 | |
<I/σ(I)> | 9.1 | 1.8 |
Completeness [%] | 92.0 | 0.648 |
Redundancy | 3.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Magnesium Acetate, MPD, cacodylate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |