1YDE
Crystal Structure of Human Retinal Short-Chain Dehydrogenase/Reductase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 167.115, 98.823, 167.463 |
| Unit cell angles | 90.00, 115.87, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.18166 |
| Rwork | 0.181 |
| R-free | 0.22903 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.505 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.117 | 0.706 |
| Number of reflections | 176307 | |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 92.0 | 0.648 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Magnesium Acetate, MPD, cacodylate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
