1YD7
Conserved hypothetical protein Pfu-1647980-001 from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 61.448, 61.448, 129.084 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.890 - 2.300 |
| R-factor | 0.20764 |
| Rwork | 0.206 |
| R-free | 0.24274 |
| Structure solution method | SAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.080 | 0.058 | 0.477 |
| Number of reflections | 17709 | ||
| Completeness [%] | 100 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 8.9 | 291 | 0.1M TRIS-HCl/NaOH, 0.5M ammonium sulfate, pH 8.9, modified microbatch, temperature 291K |
