1YCJ
Crystal structure of the kainate receptor GluR5 ligand-binding core in complex with (S)-glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8128 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.714, 57.901, 88.750 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 20.300 - 1.950 |
| R-factor | 0.20295 |
| Rwork | 0.199 |
| R-free | 0.26885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.876 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 37372 | |
| <I/σ(I)> | 18.5 | 3.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 279 | PEG4000, phosphat-citrate buffer, lithiumsulphate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
