1YCJ
Crystal structure of the kainate receptor GluR5 ligand-binding core in complex with (S)-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-11-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8128 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.714, 57.901, 88.750 |
Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
Resolution | 20.300 - 1.950 |
R-factor | 0.20295 |
Rwork | 0.199 |
R-free | 0.26885 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.021 |
RMSD bond angle | 1.876 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 37372 | |
<I/σ(I)> | 18.5 | 3.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 279 | PEG4000, phosphat-citrate buffer, lithiumsulphate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K |