1YBM

X-ray structure of selenomethionyl gene product from Arabidopsis thaliana at5g02240 in space group P21212

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-08
Detector	MAR CCD 165 mm
Wavelength(s)	0.97935, 0.97904, 0.96389
Spacegroup name	P 21 21 2
Unit cell lengths	74.634, 77.286, 92.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.090 - 2.096
Rwork	0.189
R-free	0.26150
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.643
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.087	50.000	2.150
High resolution limit [Å]	2.096	5.180	2.100
Rmerge	0.047	0.033	0.280
Total number of observations		1967	1878
Number of reflections	29937
<I/σ(I)>	22.745		6.125
Completeness [%]	93.0	84.8	88.3
Redundancy	11.7		9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 MG/ML PROTEIN, 24 PERCENT PEG 4K, 0.136 M SODIUM MALONATE, 0.10 M BISTRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K