1YAE
Structure of the Kainate Receptor Subunit GluR6 Agonist Binding Domain Complexed with Domoic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 246.360, 106.570, 172.690 |
| Unit cell angles | 90.00, 133.19, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.110 |
| R-factor | 0.27846 |
| Rwork | 0.275 |
| R-free | 0.33409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (V. 1.2) |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.500 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 58030 | |
| <I/σ(I)> | 8.7 | 2.4 |
| Completeness [%] | 97.2 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PIPES, Ammonium Sulphate, Ammonium thiocyanate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
