1Y9M
Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.42 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.816, 82.131, 136.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.890 |
| R-factor | 0.16662 |
| Rwork | 0.165 |
| R-free | 0.19973 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.307 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.054 | 0.297 |
| Number of reflections | 54756 | |
| <I/σ(I)> | 8 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% PEG3350, 1 mM sodium cacodylate, 20-100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
