1Y9K
IAA acetyltransferase from Bacillus cereus ATCC 14579
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.122, 70.122, 171.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.400 - 2.390 |
| R-factor | 0.20029 |
| Rwork | 0.198 |
| R-free | 0.23464 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.390 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 37374 | |
| <I/σ(I)> | 19.85 | |
| Completeness [%] | 99.5 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 2.0 M Ammonium sulfate, CAPS pH 10.5 0.1 Lithium Sulfate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
