1Y9E
Crystal structure of Bacillus subtilis protein yhfP with NAD bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.271, 136.922, 167.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.800 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.23300 |
Structure solution method | SAD |
RMSD bond length | 0.046 |
RMSD bond angle | 3.900 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.092 | 0.248 |
Number of reflections | 130776 | |
Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 4% PEG4000, 0.1M Bis-Tris pH 6.5, 0.16M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |