1Y98
Structure of the BRCT repeats of BRCA1 bound to a CtIP phosphopeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 113.118, 113.118, 121.876 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.590 - 2.500 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t15 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.781 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 16294 | |
| <I/σ(I)> | 17.53 | 2.68 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 11 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | AMMONIUM SULPHATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
