1Y8J

Crystal Structure of human NEP complexed with an imidazo[4,5-c]pyridine inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-08-13
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	107.355, 107.356, 112.466
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.250
R-factor	0.22984
Rwork	0.226
R-free	0.29637
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dmt
RMSD bond length	0.007
RMSD bond angle	0.889
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.390
High resolution limit [Å]	2.250	2.250
Number of reflections	34323
Completeness [%]	95.4	84.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP