1Y82
Conserved hypothetical protein Pfu-367848-001 from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97623 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.601, 103.667, 104.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.521 - 2.300 |
R-factor | 0.22346 |
Rwork | 0.222 |
R-free | 0.27790 |
Structure solution method | SAS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.381 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.109 | 0.072 | 0.349 |
Total number of observations | 3053 | 2788 | |
Number of reflections | 28211 | ||
Completeness [%] | 98.7 | 99.9 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 5.5 | 291 | 0.1M citrate, 0.1M sodium chloride, 0.1M lithium sulfate, 30% v/v PEG 400, pH 5.5, modified microbatch, temperature 291K |
1 | modified microbatch | 5.5 | 291 | 0.1M citrate, 0.1M sodium chloride, 0.1M lithium sulfate, 30% v/v PEG 400, pH 5.5, modified microbatch, temperature 291K |