1Y7V
X-ray structure of human acid-beta-glucosidase covalently bound to conduritol B epoxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.490, 285.610, 91.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.820 - 2.400 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.28000 |
| Structure solution method | Difference Fourier technique |
| Starting model (for MR) | 1ogs |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 55401 | |
| <I/σ(I)> | 12.75 | 4.88 |
| Completeness [%] | 96.4 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | Ammonium sulfate, guanidine HCl, KCl, acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
