1Y7U
Crystal Structure of Acyl-Coa hydrolase from Bacillus cereus
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 123.553, 123.553, 126.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.620 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.620 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.181 | |
| Number of reflections | 24853 | |
| <I/σ(I)> | 5 | 3.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.4 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | ammonium sulfate, PEG3350, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
