1Y7H
Structural and biochemical studies identify tobacco SABP2 as a methylsalicylate esterase and further implicate it in plant innate immunity, Northeast Structural Genomics Target AR2241
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.630, 137.736, 171.208 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 28.170 - 2.520 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.170 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.125 | 0.276 |
| Number of reflections | 138444 | |
| <I/σ(I)> | 12.18 | 4.13 |
| Completeness [%] | 98.9 | 97.5 |
| Redundancy | 4.44 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG 3350, 0.2 M sodium thiocyanate, 5 mM spermine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
