1Y79
Crystal Structure of the E.coli Dipeptidyl Carboxypeptidase Dcp in Complex with a Peptidic Inhibitor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.050, 0.97880, 0.97910, 0.95000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.408, 67.921, 153.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2403 |
| Rwork | 0.214 |
| R-free | 0.27470 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.341 |
| Number of reflections | 45498 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.2 M potassium nitrate, 18% (w/v) PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
