1Y6N

Crystal structure of Epstein-Barr virus IL-10 mutant (A87I) complexed with the soluble IL-10R1 chain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9252, 0.9794
Spacegroup name	P 32 1 2
Unit cell lengths	45.603, 45.603, 310.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.211
Rwork	0.206
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.330
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.059	0.245
Number of reflections	10500
Completeness [%]	97.6	85.1
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 6000, magnesium chloride, ADA, MPD, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K