1Y69
RRF domain I in complex with the 50S ribosomal subunit from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-14 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 168.700, 405.000, 693.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.130 - 3.330 |
| R-factor | 0.275 |
| Rwork | 0.275 |
| R-free | 0.33800 |
| Structure solution method | MR |
| Starting model (for MR) | 1nkw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 343272 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 95.2 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 290 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, pH 7.80, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ETHANOL | ||
| 10 | 1 | 2 | NH4CL | ||
| 2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | HEPES | ||
| 6 | 1 | 1 | NH4CL | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | MGCL2 | ||
| 9 | 1 | 2 | KCL |
