1Y60
Structure of the tetrahydromethanopterin dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1 with bound 5,10-methylene tetrahydromethanopterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-12-07 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.939270 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.860, 112.590, 72.010 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 44.820 - 1.900 |
| R-factor | 0.225 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Formaldehyde-activating enzyme from Methylobacterium extorquens AM1 PDB ID 1Y5Y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.820 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.144 | 0.500 |
| Number of reflections | 57191 | |
| <I/σ(I)> | 12.15 | 3.03 |
| Completeness [%] | 93.5 | 76.1 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281 | 0.1 M HEPES/NaOH pH 7.5, 20% (w/v) polyethyleneglycol 10,000, 5 mM tetrahydromethanopterin (H4MPT), VAPOR DIFFUSION, HANGING DROP, temperature 281.0K |
