1Y5Y

Structure of the tetrahydromethanopterin dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2002-07-14
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9790, 0.9792, 0.9501
Spacegroup name	P 43 21 2
Unit cell lengths	120.659, 120.659, 205.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.000
R-factor	0.221
Rwork	0.204
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.890	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	100903
<I/σ(I)>	19.3	2.88
Completeness [%]	98.6
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.2 M calciumchloride x 2H2O, 0.1 M sodium acetate x 3H2O pH 4.6 and 10-20% (v/v) isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 277K'