1Y4W
Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.954, 94.029, 67.634 |
| Unit cell angles | 90.00, 107.04, 90.00 |
Refinement procedure
| Resolution | 17.790 - 1.550 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19820 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.168 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.790 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.045 | 0.150 |
| Number of reflections | 78641 | |
| <I/σ(I)> | 10.3 | 4.5 |
| Completeness [%] | 91.0 | 85.7 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% PEG3350, 1mM sodium cacodylate, 20-100mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
