1Y4S

Conformation rearrangement of heat shock protein 90 upon ADP binding

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.9792, 0.94
Spacegroup name	P 21 21 2
Unit cell lengths	72.500, 84.180, 212.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
Rwork	0.269
R-free	0.31400
Structure solution method	MAD
RMSD bond length	0.094
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.073	0.234
Number of reflections	29771
<I/σ(I)>	14.7
Completeness [%]	100.0	100
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	16% PEG3350, 0.1M HEPES, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K